human embryonic kidney cells 293 hek293 atcc crl Search Results


95
ATCC reference identifiers additional information cell line
Reference Identifiers Additional Information Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC human embryonic kidney 293 hek293 cell lines
Human Embryonic Kidney 293 Hek293 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC hek293 cas9 rfp cells atcc crl 1573cas9 fibroblast
Hek293 Cas9 Rfp Cells Atcc Crl 1573cas9 Fibroblast, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC human embryonic kidney 293 293hek
Dose-dependent DNAm and gene expression changes following transfection of methylated DNA probes against FKBP5 . ( A ) <t>293HEK</t> cells were transfected with a single-stranded unmethylated probe (500 ng − SssI), a single-stranded methylated probe at two different concentrations (250 ng and 500 ng + SssI), or a double-stranded methylated probe (500 ng + SssI +DS). Untransfected cells served as controls (Control). DNAm levels of five CpG sites at the conserved glucocorticoid response element (GRE) of human FKBP5 intron 5 were analyzed by bisulfite pyrosequencing. Data for the first two CpGs are shown. ( B ) Typical pyrograms obtained from bisulfite pyrosequencing are shown for each group at CpG-1. The percent DNAm determination occurs when R (or A/G) is dispensed, and it corresponds to the reverse complement of T/C (T for unmethylated and C for methylation CpG, respectively). Each pyrogram represents % methylation from one sample. ( C ) FKBP5 expression was measured by qRT-PCR in the same groups of 293HEK cells as in ( A ) treated with 1 μM dexamethasone (DEX) for four hours prior to collection. Bar graphs represent the mean ± SEM, and unpaired two-tailed Student’s t -tests were performed with n = 4 per group. *** p < 0.001, ** p < 0.01, and * p < 0.05.
Human Embryonic Kidney 293 293hek, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Inno Biologics human embryonic kidney (hek 293) cells
Dose-dependent DNAm and gene expression changes following transfection of methylated DNA probes against FKBP5 . ( A ) <t>293HEK</t> cells were transfected with a single-stranded unmethylated probe (500 ng − SssI), a single-stranded methylated probe at two different concentrations (250 ng and 500 ng + SssI), or a double-stranded methylated probe (500 ng + SssI +DS). Untransfected cells served as controls (Control). DNAm levels of five CpG sites at the conserved glucocorticoid response element (GRE) of human FKBP5 intron 5 were analyzed by bisulfite pyrosequencing. Data for the first two CpGs are shown. ( B ) Typical pyrograms obtained from bisulfite pyrosequencing are shown for each group at CpG-1. The percent DNAm determination occurs when R (or A/G) is dispensed, and it corresponds to the reverse complement of T/C (T for unmethylated and C for methylation CpG, respectively). Each pyrogram represents % methylation from one sample. ( C ) FKBP5 expression was measured by qRT-PCR in the same groups of 293HEK cells as in ( A ) treated with 1 μM dexamethasone (DEX) for four hours prior to collection. Bar graphs represent the mean ± SEM, and unpaired two-tailed Student’s t -tests were performed with n = 4 per group. *** p < 0.001, ** p < 0.01, and * p < 0.05.
Human Embryonic Kidney (Hek 293) Cells, supplied by Inno Biologics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Stoelting inc human embryonal kidney cells (hek-293)
Dose-dependent DNAm and gene expression changes following transfection of methylated DNA probes against FKBP5 . ( A ) <t>293HEK</t> cells were transfected with a single-stranded unmethylated probe (500 ng − SssI), a single-stranded methylated probe at two different concentrations (250 ng and 500 ng + SssI), or a double-stranded methylated probe (500 ng + SssI +DS). Untransfected cells served as controls (Control). DNAm levels of five CpG sites at the conserved glucocorticoid response element (GRE) of human FKBP5 intron 5 were analyzed by bisulfite pyrosequencing. Data for the first two CpGs are shown. ( B ) Typical pyrograms obtained from bisulfite pyrosequencing are shown for each group at CpG-1. The percent DNAm determination occurs when R (or A/G) is dispensed, and it corresponds to the reverse complement of T/C (T for unmethylated and C for methylation CpG, respectively). Each pyrogram represents % methylation from one sample. ( C ) FKBP5 expression was measured by qRT-PCR in the same groups of 293HEK cells as in ( A ) treated with 1 μM dexamethasone (DEX) for four hours prior to collection. Bar graphs represent the mean ± SEM, and unpaired two-tailed Student’s t -tests were performed with n = 4 per group. *** p < 0.001, ** p < 0.01, and * p < 0.05.
Human Embryonal Kidney Cells (Hek 293), supplied by Stoelting inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IPS Therapeutique human embryonic kidney (hek) 293 cells transfected with a human ether-a-go-go-related gene (herg)
Dose-dependent DNAm and gene expression changes following transfection of methylated DNA probes against FKBP5 . ( A ) <t>293HEK</t> cells were transfected with a single-stranded unmethylated probe (500 ng − SssI), a single-stranded methylated probe at two different concentrations (250 ng and 500 ng + SssI), or a double-stranded methylated probe (500 ng + SssI +DS). Untransfected cells served as controls (Control). DNAm levels of five CpG sites at the conserved glucocorticoid response element (GRE) of human FKBP5 intron 5 were analyzed by bisulfite pyrosequencing. Data for the first two CpGs are shown. ( B ) Typical pyrograms obtained from bisulfite pyrosequencing are shown for each group at CpG-1. The percent DNAm determination occurs when R (or A/G) is dispensed, and it corresponds to the reverse complement of T/C (T for unmethylated and C for methylation CpG, respectively). Each pyrogram represents % methylation from one sample. ( C ) FKBP5 expression was measured by qRT-PCR in the same groups of 293HEK cells as in ( A ) treated with 1 μM dexamethasone (DEX) for four hours prior to collection. Bar graphs represent the mean ± SEM, and unpaired two-tailed Student’s t -tests were performed with n = 4 per group. *** p < 0.001, ** p < 0.01, and * p < 0.05.
Human Embryonic Kidney (Hek) 293 Cells Transfected With A Human Ether A Go Go Related Gene (Herg), supplied by IPS Therapeutique, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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293  (ATCC)
99
ATCC 293
Dose-dependent DNAm and gene expression changes following transfection of methylated DNA probes against FKBP5 . ( A ) <t>293HEK</t> cells were transfected with a single-stranded unmethylated probe (500 ng − SssI), a single-stranded methylated probe at two different concentrations (250 ng and 500 ng + SssI), or a double-stranded methylated probe (500 ng + SssI +DS). Untransfected cells served as controls (Control). DNAm levels of five CpG sites at the conserved glucocorticoid response element (GRE) of human FKBP5 intron 5 were analyzed by bisulfite pyrosequencing. Data for the first two CpGs are shown. ( B ) Typical pyrograms obtained from bisulfite pyrosequencing are shown for each group at CpG-1. The percent DNAm determination occurs when R (or A/G) is dispensed, and it corresponds to the reverse complement of T/C (T for unmethylated and C for methylation CpG, respectively). Each pyrogram represents % methylation from one sample. ( C ) FKBP5 expression was measured by qRT-PCR in the same groups of 293HEK cells as in ( A ) treated with 1 μM dexamethasone (DEX) for four hours prior to collection. Bar graphs represent the mean ± SEM, and unpaired two-tailed Student’s t -tests were performed with n = 4 per group. *** p < 0.001, ** p < 0.01, and * p < 0.05.
293, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Rocha labs human embryonic kidney (hek) 293 cell lines
Dose-dependent DNAm and gene expression changes following transfection of methylated DNA probes against FKBP5 . ( A ) <t>293HEK</t> cells were transfected with a single-stranded unmethylated probe (500 ng − SssI), a single-stranded methylated probe at two different concentrations (250 ng and 500 ng + SssI), or a double-stranded methylated probe (500 ng + SssI +DS). Untransfected cells served as controls (Control). DNAm levels of five CpG sites at the conserved glucocorticoid response element (GRE) of human FKBP5 intron 5 were analyzed by bisulfite pyrosequencing. Data for the first two CpGs are shown. ( B ) Typical pyrograms obtained from bisulfite pyrosequencing are shown for each group at CpG-1. The percent DNAm determination occurs when R (or A/G) is dispensed, and it corresponds to the reverse complement of T/C (T for unmethylated and C for methylation CpG, respectively). Each pyrogram represents % methylation from one sample. ( C ) FKBP5 expression was measured by qRT-PCR in the same groups of 293HEK cells as in ( A ) treated with 1 μM dexamethasone (DEX) for four hours prior to collection. Bar graphs represent the mean ± SEM, and unpaired two-tailed Student’s t -tests were performed with n = 4 per group. *** p < 0.001, ** p < 0.01, and * p < 0.05.
Human Embryonic Kidney (Hek) 293 Cell Lines, supplied by Rocha labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cyprotex Discovery human embryonic kidney hek 293 cells
Dose-dependent DNAm and gene expression changes following transfection of methylated DNA probes against FKBP5 . ( A ) <t>293HEK</t> cells were transfected with a single-stranded unmethylated probe (500 ng − SssI), a single-stranded methylated probe at two different concentrations (250 ng and 500 ng + SssI), or a double-stranded methylated probe (500 ng + SssI +DS). Untransfected cells served as controls (Control). DNAm levels of five CpG sites at the conserved glucocorticoid response element (GRE) of human FKBP5 intron 5 were analyzed by bisulfite pyrosequencing. Data for the first two CpGs are shown. ( B ) Typical pyrograms obtained from bisulfite pyrosequencing are shown for each group at CpG-1. The percent DNAm determination occurs when R (or A/G) is dispensed, and it corresponds to the reverse complement of T/C (T for unmethylated and C for methylation CpG, respectively). Each pyrogram represents % methylation from one sample. ( C ) FKBP5 expression was measured by qRT-PCR in the same groups of 293HEK cells as in ( A ) treated with 1 μM dexamethasone (DEX) for four hours prior to collection. Bar graphs represent the mean ± SEM, and unpaired two-tailed Student’s t -tests were performed with n = 4 per group. *** p < 0.001, ** p < 0.01, and * p < 0.05.
Human Embryonic Kidney Hek 293 Cells, supplied by Cyprotex Discovery, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Dose-dependent DNAm and gene expression changes following transfection of methylated DNA probes against FKBP5 . ( A ) 293HEK cells were transfected with a single-stranded unmethylated probe (500 ng − SssI), a single-stranded methylated probe at two different concentrations (250 ng and 500 ng + SssI), or a double-stranded methylated probe (500 ng + SssI +DS). Untransfected cells served as controls (Control). DNAm levels of five CpG sites at the conserved glucocorticoid response element (GRE) of human FKBP5 intron 5 were analyzed by bisulfite pyrosequencing. Data for the first two CpGs are shown. ( B ) Typical pyrograms obtained from bisulfite pyrosequencing are shown for each group at CpG-1. The percent DNAm determination occurs when R (or A/G) is dispensed, and it corresponds to the reverse complement of T/C (T for unmethylated and C for methylation CpG, respectively). Each pyrogram represents % methylation from one sample. ( C ) FKBP5 expression was measured by qRT-PCR in the same groups of 293HEK cells as in ( A ) treated with 1 μM dexamethasone (DEX) for four hours prior to collection. Bar graphs represent the mean ± SEM, and unpaired two-tailed Student’s t -tests were performed with n = 4 per group. *** p < 0.001, ** p < 0.01, and * p < 0.05.

Journal: International Journal of Molecular Sciences

Article Title: Targeted DNA Methylation Using Modified DNA Probes: A Potential Therapeutic Tool for Depression and Stress-Related Disorders

doi: 10.3390/ijms26125643

Figure Lengend Snippet: Dose-dependent DNAm and gene expression changes following transfection of methylated DNA probes against FKBP5 . ( A ) 293HEK cells were transfected with a single-stranded unmethylated probe (500 ng − SssI), a single-stranded methylated probe at two different concentrations (250 ng and 500 ng + SssI), or a double-stranded methylated probe (500 ng + SssI +DS). Untransfected cells served as controls (Control). DNAm levels of five CpG sites at the conserved glucocorticoid response element (GRE) of human FKBP5 intron 5 were analyzed by bisulfite pyrosequencing. Data for the first two CpGs are shown. ( B ) Typical pyrograms obtained from bisulfite pyrosequencing are shown for each group at CpG-1. The percent DNAm determination occurs when R (or A/G) is dispensed, and it corresponds to the reverse complement of T/C (T for unmethylated and C for methylation CpG, respectively). Each pyrogram represents % methylation from one sample. ( C ) FKBP5 expression was measured by qRT-PCR in the same groups of 293HEK cells as in ( A ) treated with 1 μM dexamethasone (DEX) for four hours prior to collection. Bar graphs represent the mean ± SEM, and unpaired two-tailed Student’s t -tests were performed with n = 4 per group. *** p < 0.001, ** p < 0.01, and * p < 0.05.

Article Snippet: Human embryonic kidney 293 (293HEK) and mouse pituitary AtT-20 cells were purchased from Atcc.org and were treated with 1 μM dexamethasone (DEX) for 5 days and cultured for an additional 5 days without any DEX.

Techniques: Gene Expression, Transfection, Methylation, Control, Expressing, Quantitative RT-PCR, Two Tailed Test

Persistence and accumulation of DNA methylation. ( A ) 293HEK cells were transfected with the unmethylated (−SssI) and methylated (+SssI) FKBP5 DNA probe at Week 1 (W1), after which the cells were cultured for an additional four weeks (W4) before analysis by bisulfite pyrosequencing. ( B ) 293HEK cells were transfected with unmethylated (−SssI) and methylated (+SssI) FKBP5 DNA probes consecutively every three days and expanded, while 50% of the cells were collected for analysis. Bar graphs represent the mean ± SEM, and unpaired two-tailed Student’s t -tests were performed with n = 4 per group. *** p < 0.001, ** p < 0.01, and * p < 0.05.

Journal: International Journal of Molecular Sciences

Article Title: Targeted DNA Methylation Using Modified DNA Probes: A Potential Therapeutic Tool for Depression and Stress-Related Disorders

doi: 10.3390/ijms26125643

Figure Lengend Snippet: Persistence and accumulation of DNA methylation. ( A ) 293HEK cells were transfected with the unmethylated (−SssI) and methylated (+SssI) FKBP5 DNA probe at Week 1 (W1), after which the cells were cultured for an additional four weeks (W4) before analysis by bisulfite pyrosequencing. ( B ) 293HEK cells were transfected with unmethylated (−SssI) and methylated (+SssI) FKBP5 DNA probes consecutively every three days and expanded, while 50% of the cells were collected for analysis. Bar graphs represent the mean ± SEM, and unpaired two-tailed Student’s t -tests were performed with n = 4 per group. *** p < 0.001, ** p < 0.01, and * p < 0.05.

Article Snippet: Human embryonic kidney 293 (293HEK) and mouse pituitary AtT-20 cells were purchased from Atcc.org and were treated with 1 μM dexamethasone (DEX) for 5 days and cultured for an additional 5 days without any DEX.

Techniques: DNA Methylation Assay, Transfection, Methylation, Cell Culture, Two Tailed Test

DNAm and gene expression analysis following transfection of methylated DNA probes against MAOA . ( A ) 293HEK cells were transfected with a single-stranded unmethylated probe (-SssI) or a single-stranded methylated probe (+SssI). DNAm levels of 14 CpG sites at a regulatory region of human MAOA were analyzed by bisulfite pyrosequencing. ( B ) MAOA expression was measured by qRT-PCR in the same groups of 293HEK cells. Bar graphs represent the mean ± SEM, and unpaired two-tailed Student’s t -tests were performed with n = 4 per group. * p < 0.05 and ** p < 0.01. A one-way ANOVA was used to test for overall differences across all 14 CpGs, followed by Tukey’s post hoc test for pairwise comparisons.

Journal: International Journal of Molecular Sciences

Article Title: Targeted DNA Methylation Using Modified DNA Probes: A Potential Therapeutic Tool for Depression and Stress-Related Disorders

doi: 10.3390/ijms26125643

Figure Lengend Snippet: DNAm and gene expression analysis following transfection of methylated DNA probes against MAOA . ( A ) 293HEK cells were transfected with a single-stranded unmethylated probe (-SssI) or a single-stranded methylated probe (+SssI). DNAm levels of 14 CpG sites at a regulatory region of human MAOA were analyzed by bisulfite pyrosequencing. ( B ) MAOA expression was measured by qRT-PCR in the same groups of 293HEK cells. Bar graphs represent the mean ± SEM, and unpaired two-tailed Student’s t -tests were performed with n = 4 per group. * p < 0.05 and ** p < 0.01. A one-way ANOVA was used to test for overall differences across all 14 CpGs, followed by Tukey’s post hoc test for pairwise comparisons.

Article Snippet: Human embryonic kidney 293 (293HEK) and mouse pituitary AtT-20 cells were purchased from Atcc.org and were treated with 1 μM dexamethasone (DEX) for 5 days and cultured for an additional 5 days without any DEX.

Techniques: Gene Expression, Transfection, Methylation, Expressing, Quantitative RT-PCR, Two Tailed Test